Safety Risks: Minimal
The air pumps make a loud noise and lithium ion batteries require care when handling. Roses have sharp prickles, wear gloves to protect from scratches. Hearing protection is recommended. There may be a risk of electric shock in rainy conditions. Wear appropriate protection from the sun: sunscreen and water are recommended.
Equipment:
Clean air system:
- 3 high volume air inflators (24v, 25 CFM) w/ nozzle attachments
- 6-8 lithium ion (24v, 4.0 Ah) power tool batteries (charged)
- 2 variable flow meters with PTFE tubing
- 2 In line Hepa Sanitary Air Filters for 1/4 in tubing (attached to flow meter)
- 2 inline water filter carbon blocks, one micron filtration
Headspace volatile collection equipment:
- GoBeGreen Original Garden Bags - Nylon - One per sample + Backups (precooked in oven at 50 C for 25 hours or more)
- Zip ties - 6 + per each sample, bring a large number
- Heavy duty shears (for cutting zip ties and holes in the bags)
- Bypass pruners (optional, for removing dead canes, allowing better access to rose flowers)
- Blue mini inline filters (for outflow)
Eqiupment for Internal Standard:
- Nonyl acetate (keep cool)
- 1 Drummond Scientific Aspirator, Captrol III
- 5 ul glass calibrated micropipettes
Experimental Site:

Sample Selection:
For this experiment, we will be collecting headspace volatiles with Solid Phase Microextraction (SPME), a method which adsorbs the volatile organic compounds (VOCs) surrounding part of a plant. It is a flexible method which can collect the VOCs from just about anything you can get an oven bag around. We want to collect the volatiles emanating from roses with various treatments, including:
- roses infested with eriophyid mites (plots: 5, 9, 24, 18)
- roses treated with Acibenzolar-S-methyl (ASM, Actigard) (plots: 1, 6, 22, 17)
- roses afflicted with Rose Rosette Disease (already sampled in Athens, GA)
To begin, select a cluster of flowers from a single rose cane, and gently cover it with an oven bag. Seal the end of the oven bag over the base of the rose cane with a zip tie so that the air is trapped around the
Notes and Figures: